Its extracts show significant antimicrobial activity against staphylococcus aureus, proteus vulgaris, pseudomonas aeruginosa, escherichia coli, aspergillus niger and fusarium species 21. The antibacterial activity reveals that the methanolic extract of datura stramonium seeds is highly active against both gram positive and gram. Its extracts show significant antimicrobial activity against staphylococcus aureus, proteusvulgaris, pseudomonasaeruginosa, escherichia coli, aspergillus niger and fusariumspecies. Little or no antimicrobial activity was found against escherichia coli and psuedomonas aeruginosa. Antibacterial compounds in different species of datura a. The results of agar well diffusion assay indicated that the pattern of inhibition depends largely. Joshi and kaur 20, worked on antimicrobial activity of ethanol, methanol and aqueous extract of d.
Datura stramonium could be potential sources of chemotherapeutic agents. Antimicrobial activity of polyphenols and alkaloids in. Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas. Comparative antimicrobial activity and phytochemical analysis of datura stramonium l. The aim of this study was to assess the compounds of alkaloids extracts from the leaves of datura stramonium, which can be the basis for the synthesis of new antibiotics. Datura, jimson weed datura stramonium, packet of 50. Antimicrobial activity of datura innoxia and datura. We are still open for nursery plant sales, and look forward to your live root and plant orders. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of 1. The aqueous extract of the plant displayed antioxidant activity of between 49.
Manjunath department of biochemistry, central college campus, bangalore university. Phytochemical and antimicrobial property of datura stramonium. The present study was conducted to identify the preliminary phytochemical identification and antimicrobial investigation of leaves extracts of datura stramonium linn collected from eastern ethiopia. Analysis of alkaloid phytochemical compounds in the. Aerial part mainly stem and bark of datura stramonium s aqueous and ethanolic extract opposite to eschericia coli. The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of datura metel, datura innoxia and brugmansia suaveolens. Antimicrobial activities of methanolic extract of datura stramonium seeds material and methods. The dried seeds of datura stramonium which were obtained from seed pods of datura plant. The antibacterial activity of the methanol extracts of the aerial parts of the datura innoxia and datura stramonium was investigated.
Plant tissue homogenization and extract preparation. Besides its hallucinogen activity, datura reported to have antimicrobial activity rajesh and sharma, 2002. Antimicrobial activity of datura innoxia and datura stramonium. Little or no antibacterial activity was found against escherichia coli and pseudomonas aeruginosa. Comparative bacterial inhibition by bioactive extracts. Paramedical college, dehradun248 991, india department of environmental science, graphic era university. Datura stramonium is commonly known as jimson weed and is widely used in phytomedicine to treat epilepsy, skin ulcers, etc as well as to kill the microbes associated with wound lesions. Datura stramonium, crude extract, phytochemical screening, antimicrobial activity, paper disk diffusion method. Ethanolic extract showed more antibacterial activity than the aqueous extract. The acceptance of traditional medicines as an alternative form of health care has led researchers to investigate the antimicrobial activity of medicinal plants 2, 3. The aim of the present study was to investigate the antimicrobial activity and phytochemical screening. Antimicrobial activity of cotton and silk fabrics dyed with datura stramonium jimson weed plant leaf extracts alex musinguzi department of manufacturing, industrial and textile engineering, school of engineering, p. An extract of datura stramonium was screened for phytochemical analysis and antimicrobial assay against different bacterial species. The main objective of this study was to evaluate the phytochemical composition, antioxidant, antifungal and insecticidal activities of aqueous and methanolic extracts.
Pdf antimicrobial investigation of datura stramonium leaf. The general results observed consider the plant as natural source of antioxidants and phytochemical quality for. Antioxidant and antimicrobial properties of seed oil of. Chemical tests and spectrophotometric methods were used for antioxidant activity. Antimicrobial activity of carica papaya, piper nigrum and. Datura stramonium is traditionally used to cure different human diseases including in skin disorder, ear pain, cough, fever, burns, and asthma in ethiopia. Antimicrobial activity seed oil datura metel jebtm authors info r. Nematicidal activity of aqueous leaf extracts of datura. Test organism the microorganism used for this study, bacterium.
College of arts, science and commerce, chembur, mumbai 400071, india. Richa bhardwaj, ankita yadav, pallavi sharma and ram avatar sharma, 2014. Datura stramonium and phytolacca dodecandra in different solvents. Datura stramonium the antimicrobial activity of the aqueous and ethanolic extract of the stembark of datura stramonium was investigation against staphylococcus aureus, salmonella typhi, shigella spp, eschericia coli, klebsiella pneumonia and neisseria gonorrhea. Datura stramonium linn solanaceae grows as a wasteland weed 4, 5 mostly in the tropical and temperate areas of the world. Phytochemical screening and antimicrobial assessment of. The bacterium streptococcus mutants was resistant to methanol extract while the fungus candida albicans was resistant to methanol and acetone extract. Antimicrobial activity of cotton and silk fabrics dyed. It is 60120 cm or more tall, branched, and pubescent plant. Comparative antimicrobial activity and phytochemical. Leaves, seeds and roots contain the alkaloid daturine.
Introduction datura stramonium is an annual herb, with stem erect and spreading branches above. Introduction datura species can be found throughout the world, except the colder or arctic regions, it grows wild in all of the worlds warm and moderate region and it is very distributed in the temperate and tropical areas 18. Phytochemical and antimicrobial property of datura stramonium 140 pestle. In vitro evaluation of datura innoxia thornapple for. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university, vellore632 014, tamil nadu, india. Antimicrobial activity of some common indian medicinal plants against some selective human pathogen h. Antimicrobial activity of ethyl acetate and methanol extracts of leaves and flowers of datura stramonium was investigated against antibiotic. Chemical constituents and pharmacological properties of. All datura plants contain tropane alkaloid such as hyoscyamine, scopolamine and atropine in the seeds and the flowers.
Fungal endophytes were isolated from surface sterilized aerial parts of d. Extraction and purification of antimicrobial proteins from. Zoology department, samrat prithviraj chauhan govt. Antibacterial, phytochemical and antioxidant activities of. Antibacterial and antifungal activity of different extracts of datura. Various parts of datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against gramnegative bacteria escherichia coli and salmonella typhi and grampositive bacteria bacillus cereus, bacillus subtilis and staphylococcus aureus. Datura stramonium is a natural source of antioxidants and phytochemical having antimicrobial activities.
Antimicrobial activity of some common indian medicinal. Its diverse biological activities include antiasthmatic, antibacterial, antifungal, antiinflammatory, larvacidal. Joshi 11 23 1 2 3 department of chemistry, graphic era university, dehradun248 002, india department of biotechnology, doon p. Datura stramonium plants on drug resistant pathogens isolated from clinical specimens dr. Antibacterial activity of the plant extract and determination of minimum inhibitory concentration. It is common in the waste land, fields and gardens in baghdad district figure 1.
Comparative bacterial inhibition by bioactive extracts from datura. Callus induction and antimicrobial activities of callus. Datura stramonium was examined for its antimicrobial activity on some oral pathogens using methanol, chloroform, acetone and water as extractants. Datura stramonium is an annual herbaceous plant of the solanaceae family. In vitro and in vivo gcms profile and antimicrobial activity of phytosterols of datura stramonium. Alkaloids, antibacterial activity, datura stramonium, gas chromatographymass specroscopy. Nagesh malik1, samreen ahmed2 1,2 department of microbiology, v. Comparative antimicrobial activity and phytochemical analysis of.
Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas aeruginosa, agrobacterium tumefaciens and klebsiella. Home shop datura, jimson weed datura stramonium, packet of 50 seeds, organic we at strictly medicinal seeds are dedicated to providing medicinal plants to the people. Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. The extraction was carried out using trishcl buffer prepared from 50m tris and 0. Datura stramonium extract, zone of inhibition, antibacterial activity, antifungal activity, agar well diffusion method, minimum inhibitory concentration. Little or no antibacterial activity was found against escherichia coli and. Comparative bacterial inhibition by bioactive extracts from datura stramonium and phytolacca dodecandra. Pdf antimicrobial investigation of datura stramonium. These dried seeds were pulverized using sterile laboratory mortar and.
Abstract the phytochemical analysis and antimicrobial activities of in vitro grown callus and in d. The fine powder were collected into a sterile aluminum foil and kept in a cool dry place till further use. The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of adhatoda vasica, azadirachta indica and datura stramonium leaf extract. Dried plant leaves of datura stramonium, carica papaya and fruits of piper nigrum. Abstract datura stramonium is a plant of medicinal plant family solananceae. This study is conducted to investigate the antimicrobial property of proteins present in the seeds of datura stramonium. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts. Antimicrobial property of datura leaf extract against. Datura stramonium solanaceae is an important medicinal plant from which tropane alkaloids, amino acids, tannin, phytic acids, carbohydrates have been isolated. Phytochemical screening and antimicrobial assessment of abutilon. The secondary metabolites of datura stramonium are highly effective against different types of diseases such as antdiabetic, antiviral, etc. Natural dyes and its antimicrobial effect smita bhuyan, dr.
In this study, crude extracts from fungal endophytes isolated from datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. Pdf antimicrobial property of datura leaf extract against methicillin. Datura stramonium, callus induction, antibacterial activity, antifungal activity, phytochemical analysis. In vitro and in vivo gcms profile and antimicrobial. Evaluation of acute and subacute toxicity of alkaloids. Pdf analysis of alkaloid phytochemical compounds in the.
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